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Mohammed Ali Ahmed Saeed Abdul Hakeem Memon Armaghan Shafaei Moh'd Shahrul Ridzuan Hamil Amirin Sadikun Zhari Ismail

Abstract

Background: Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila has been used traditionally for the treatment of several ailments such as gonorrhoea, dysmenorrhoea
and as tonics for females females.
Aims
Aims: To investigate the standardization procedure of Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila extract (LPE) and evaluation of its nanoliposomes.
Methods:
Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila Labisia pumila extract was standardized by high performance liquid chromatography (HPLC), and gallic acid, caffeic
acid, rutin and 2,4 2,4-Di -tert tert-butylphenol were quantified in the extract. Then, the standardized extract was prepared as nanoliposome
using soy bean phospholipid by the film method and after that was characterized by zetasizer, zeta potential, UV -Vis
spectrophotometer and FTIR techniques techniques.
Results:
For the standardization, the mean percentage recovery values of the concentration studied were 98.49 98.49±1.43, 97.01 97.01±2.04,
97.7097.70±1.55 and 99.43 99.43±3.04 % for gallic acid, caffeic acid, rutin and 2,42,4-Di -tert tert-butylphenol, respectively. The accuracy values were
between 95.06 and 104.86% for the marker compounds, while the corresponding precision values were betwee n 0.09 and 5.18% for
withinwithin-day and between between-day analysis, respectively. The average particle size for LLP was 174.20±4.58 nm with zeta potential of
particles surface charge from −43.40 to − 44.40 mV. The polydispersity index was 0.19±0.02 and the morpholo gy and presence of
liposomes were further confirmed by transmission electron microscopy which revealed the presence of spherical liposomes of < 200
nm.
Conclusion:
HPLC method for the simultaneous determination of selected marker compounds has been develop ed; the method was
reliable, repeatable and reproducible. The method was successfully applied in standardization of LPE. LPE was successfully pr epared
as nanoliposome using soybean phospholipid.

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Keywords

pumilaLabisia pumilaLabisia pumilaLabisia pumila Labisia pumila Labisia pumilaLabisia pumilaLabisia pumilaLabisia pumila Labisia pumila, standardization, nano liposome, gal lic acid, caffeic acid, rutin, high performance liquid chromatography, phospholipid, soybean, zetasizer, particle size, zeta potential

Section
Original Article