The HPLC And FTIR Analytical Techniques To Determination of Para-phenylenediamine In Some Black Hair Dyes

Abstract

Para-phenylenediamine (PPD) is a chemical compound commonly, used in the dyeing industry, particularly in hair coloring products. PPD poses potential health risks to humans, cause respiratory problems, asthma, allergic reactions, leading to skin irritation, rashes and potential carcinogenic effects during dealing, using or exposure. The aim of the present study uses a rapid, develop. suitable sensitive analytical method for stability studies to investigates PPD by using two analytical methods: High-Performance Liquid Chromatography (HPLC) with UV detection and Fourier Transform Infrared (FTIR) spectrum to confirm the identification of its component PPD in some dying hair samples that use in Yemen. FTIR analysis was employed to identify characteristic functional groups associated with PPD in the dye matrix, providing qualitative information about its presence. The spectral data illustrated that N-H aromatic stretching various peaks in   Black Stone Dye, Brand-1, Brand-2 and Brand-3 samples to different PPD concentrations or the presence of impurities, and additional materials in samples collected. HPLC utilized for qualitative and quantitative analysis of PPD in Black Henna samples, where the result HPLC analysis an appropriate method to achieve optimal separation of PPD from other components in the dye samples. Calibration curves were established using known concentrations of PPD, enabling accurate quantification of PPD in Black Henna samples at concentrations 1.9% for Brand-2, Brand-1 (6.98%), Brand-3 (14.33%) and Black Stone (70.35%) that make the three samples higher than permissible levels in black henna samples. The results demonstrate that the combined use of FTIR and HPLC offers a robust methodology for the detection and quantification of PPD in black henna samples that integrated approach can facilitate better monitoring of PPD levels, contributing to improved consumer safety and environmental protection.